3WBD
Crystal structure of anti-polysialic acid antibody single chain Fv fragment (mAb735) complexed with octasialic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 64 |
Unit cell lengths | 161.174, 161.174, 45.884 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 100.000 - 1.800 |
R-factor | 0.23446 |
Rwork | 0.233 |
R-free | 0.25883 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1plg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 63488 | |
<I/σ(I)> | 40.1 | 6.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.2 | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.8M Ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |