3WB8
Crystal Structure of MyoVa-GTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Collection date | 2011-04-26 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.984, 165.355, 173.020 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 41.793 - 2.499 |
| R-factor | 0.1756 |
| Rwork | 0.173 |
| R-free | 0.22530 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.499 |
| Rmerge | 0.067 |
| Number of reflections | 131888 |
| <I/σ(I)> | 22.64 |
| Completeness [%] | 98.9 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20-30%(w/v) ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
