3WB8
Crystal Structure of MyoVa-GTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Collection date | 2011-04-26 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.984, 165.355, 173.020 |
Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
Resolution | 41.793 - 2.499 |
R-factor | 0.1756 |
Rwork | 0.173 |
R-free | 0.22530 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.064 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.499 |
Rmerge | 0.067 |
Number of reflections | 131888 |
<I/σ(I)> | 22.64 |
Completeness [%] | 98.9 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20-30%(w/v) ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |