3WAN
Crystal structure of Atg13 LIR-fused human LC3A_2-121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-01-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.730, 47.880, 77.080 |
| Unit cell angles | 90.00, 94.22, 90.00 |
Refinement procedure
| Resolution | 47.880 - 1.770 |
| R-factor | 0.17643 |
| Rwork | 0.174 |
| R-free | 0.22675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vtu |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.145 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.880 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.059 | 0.194 |
| Number of reflections | 24414 | |
| <I/σ(I)> | 12.6 | 4.9 |
| Completeness [%] | 99.0 | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 12% MPD, 0.1M Sodium chloride, 0.1M Sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
