3WAK

Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-27
Detector	RAYONIX MX325HE
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	123.357, 123.357, 182.496
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.896 - 3.413
R-factor	0.2213
Rwork	0.219
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3waj
RMSD bond length	0.003
RMSD bond angle	0.787
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.900	3.460
High resolution limit [Å]	3.400	3.410
Rmerge	0.151
Number of reflections	19650
<I/σ(I)>	15.8	2.5
Completeness [%]	99.5	100
Redundancy		13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.02M Tris-HCl, 22% PEG 550MME, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K