3WAG

Crystal structure of glycosyltransferase VinC in complex with DTDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-29
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	98.829, 130.162, 139.787
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.750 - 2.100
R-factor	0.194
Rwork	0.191
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wad
RMSD bond length	0.017
RMSD bond angle	2.029
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC (5.6.0117)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	48895
Completeness [%]	93.0	74.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20 %(W/V) PEG 3350, 0.2M MGCL2, 100MM TRIS, 10MM DTDP, PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K