3WAD
Crystal structure of glycosyltransferase VinC involved in the biosynthesis of vicenistatin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-30 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0, 0.97906, 0.97938, 0.96415 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 98.205, 130.392, 140.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.210 - 2.000 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.25300 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.977 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.980 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 60204 | |
| Completeness [%] | 98.5 | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20%(W/V) PEG 3350, MGCL2, TRIS, HCL, PH 8.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
