3WAB
Carboxypeptidase B in complex with DD2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 86 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-29 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.541865 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 79.314, 79.314, 100.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.154 |
| Rwork | 0.207 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zg7 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 17871 | |
| Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 296 | 100mM sodium cacodylate (pH 5.3-6.8), 100mM zinc acetate, 5-15% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
