3WA9
The nucleosome containing human H2A.Z.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.903, 109.390, 181.764 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.963 - 3.070 |
R-factor | 0.2248 |
Rwork | 0.222 |
R-free | 0.27070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.008 |
RMSD bond angle | 1.315 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.180 |
High resolution limit [Å] | 3.070 | 6.610 | 3.070 |
Number of reflections | 40504 | ||
<I/σ(I)> | 12.3 | 62.7 | 4.5 |
Completeness [%] | 99.8 | 98 | 100 |
Redundancy | 6.3 | 6.3 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |