3W9S

Crystal Structure Analysis of the N-terminal Receiver domain of Response Regulator PmrA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	293
Detector technology	CCD
Collection date	2008-10-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97316
Spacegroup name	P 41
Unit cell lengths	49.994, 49.994, 145.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.380 - 1.700
R-factor	0.1657
Rwork	0.165
R-free	0.17530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zes
RMSD bond length	0.006
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.380	30.000
High resolution limit [Å]	1.700	1.700
Number of reflections	38222
Completeness [%]	95.1	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100mM Imidazole, 1M sodium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K