3W9R
Crystal structure of the high-affinity abscisic acid receptor PYL9/RCAR9 bound to ABA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 111.405, 111.405, 40.478 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.18893 |
Rwork | 0.188 |
R-free | 0.20447 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cnw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.265 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.570 |
Number of reflections | 23009 | |
<I/σ(I)> | 60.7 | 4.73 |
Completeness [%] | 99.8 | 100 |
Redundancy | 10.7 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 16.5 (w/v)% PEG 1500, maric acid-MES-Tris (MMT) buffer, pH 7.0, VAPOR DIFFUSION, temperature 293K |