3W94
Structure of Oryzias latipes enteropeptidase light chain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 200 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.470, 71.641, 134.433 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.145 - 2.002 |
R-factor | 0.1954 |
Rwork | 0.193 |
R-free | 0.24090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.221 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 32262 | |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 291 | 20% PEG 3350, 0.1M sodium acetate trihydrate, 0.1M cadmium chloride hydrate, pH 4.6, VAPOR DIFFUSION, temperature 291.0K |