3W8Z
The complex structure of EncM with hydroxytetraketide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.070, 85.120, 79.340 |
| Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
| Resolution | 42.600 - 1.800 |
| R-factor | 0.15978 |
| Rwork | 0.158 |
| R-free | 0.19581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w8w |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.161 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 86217 | |
| Completeness [%] | 99.9 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 2mM DTT, 0.1M HEPES-Na (pH 7.5), 0.2M calcium acetate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
