3W8S
Crystal structure of monomeric Na-GST-3, a glutathione s-transferase from the major human hookworm parasite Necator americanus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 103.15 |
Detector technology | IMAGE PLATE |
Collection date | 2013-03-16 |
Detector | RIGAKU RAXIS HTC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.120, 67.120, 134.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.650 - 2.070 |
R-factor | 0.1743 |
Rwork | 0.172 |
R-free | 0.22014 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2on7 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.006 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.740 | 2.240 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.143 | 0.470 |
Number of reflections | 19536 | |
<I/σ(I)> | 17.8 | 0.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.1 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 298 | 0.08M sodium acetate trihydrate pH 4.6, 20% (w/v) PEG 4000, 0.16M ammonium sulfate, 20% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |