3W80
Crystal structure of dodecamer human insulin with double C-axis length of the hexamer 2 Zn insulin cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6C |
| Synchrotron site | Photon Factory |
| Beamline | BL-6C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-05 |
| Detector | Fully automatic high speed Weissenberg data collection system called Galaxy |
| Wavelength(s) | 1.00 |
| Spacegroup name | H 3 |
| Unit cell lengths | 82.720, 82.720, 68.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.18539 |
| Rwork | 0.182 |
| R-free | 0.24845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w7y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.258 |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.000 |
| Rmerge | 0.055 |
| Number of reflections | 90240 |
| Redundancy | 4.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.67 | 293 | 10mg/ml protein, 0.1M Sodium Citrate, 22%(v/v) DMF, 0.08%(w/v) Zinc chloride, pH 8.67, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
