3W6W
Crystal structure of melB holo-protyrosinase from Asperugillus oryzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.313, 118.086, 84.207 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 34.074 - 1.394 |
| R-factor | 0.1785 |
| Rwork | 0.177 |
| R-free | 0.19920 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.102 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX ((AutoSol)) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.400 |
| Number of reflections | 198757 | |
| Completeness [%] | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 18% PEG 3350, 50mM Ammonium Fluoride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
