3W6W
Crystal structure of melB holo-protyrosinase from Asperugillus oryzae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-07 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.313, 118.086, 84.207 |
Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
Resolution | 34.074 - 1.394 |
R-factor | 0.1785 |
Rwork | 0.177 |
R-free | 0.19920 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.102 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX ((AutoSol)) |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.380 | 1.400 |
Number of reflections | 198757 | |
Completeness [%] | 96.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 18% PEG 3350, 50mM Ammonium Fluoride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |