3W6U
Crystal structure of NADP bound L-serine 3-dehydrogenase from Hyperthermophilic Archaeon Pyrobaculum calidifontis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-30 |
Detector | Pilatus 2M-F |
Wavelength(s) | 0.9789, 0.9792, 0.9640 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.603, 56.827, 56.571 |
Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
Resolution | 39.750 - 2.000 |
Rwork | 0.227 |
R-free | 0.24200 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 25316 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% PEG2000, 0.2 M ammonium sulfate, 0.1M acetate buffer , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |