3W6A
Crystal structure of cross-linked tetragonal hen egg white lysozyme soaked wiht 5mM [Ru(benzene)Cl2]2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.046, 79.046, 36.358 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.950 - 1.770 |
R-factor | 0.20188 |
Rwork | 0.199 |
R-free | 0.27201 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.412 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.820 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.047 | |
Number of reflections | 29518 | |
Completeness [%] | 99.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | sodium chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |