3W65
MamM-CTD D249A and H264A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5417 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.913, 94.476, 53.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.970 - 2.370 |
| R-factor | 0.1936 |
| Rwork | 0.191 |
| R-free | 0.24470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.713 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 | 2.410 |
| High resolution limit [Å] | 2.370 | 6.400 | 2.370 |
| Rmerge | 0.071 | 0.054 | 0.408 |
| Number of reflections | 4059 | ||
| <I/σ(I)> | 12.4 | ||
| Completeness [%] | 99.9 | 100 | 99 |
| Redundancy | 8.1 | 7.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M BIS-TRIS PH=5.5, 25% PEG 3350, 0.2M AmSO4 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
