3W5Y
MamM-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 133.384, 133.384, 133.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.680 - 1.950 |
| R-factor | 0.2096 |
| Rwork | 0.207 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.863 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.260 | 1.950 |
| Rmerge | 0.062 | 0.043 | 0.434 |
| Number of reflections | 13839 | ||
| <I/σ(I)> | 20.7 | ||
| Completeness [%] | 96.1 | 83.1 | 100 |
| Redundancy | 5.6 | 5.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 7.5% 2-propanol 1.75M AmSO4 , pH 8, VAPOR DIFFUSION, SITTING DROP |
