3W5N
Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-03-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.063, 128.559, 75.260 |
Unit cell angles | 90.00, 99.85, 90.00 |
Refinement procedure
Resolution | 40.560 - 1.800 |
R-factor | 0.16442 |
Rwork | 0.163 |
R-free | 0.19366 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w5m |
RMSD bond length | 0.009 |
RMSD bond angle | 1.259 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.7.0029) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.590 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 91813 | |
<I/σ(I)> | 27.5 | 3.9 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.6 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 10% (w/v) PEG 6000, 5% (v/v) MPD, 0.1M HEPES, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |