3W5G
Crystal structure of tomato beta-galactosidase 4 in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.930, 106.382, 162.379 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.060 - 3.000 |
| R-factor | 0.20274 |
| Rwork | 0.200 |
| R-free | 0.25908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5f |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.097 | 0.363 |
| Number of reflections | 32837 | |
| <I/σ(I)> | 22.2 | 3.5 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 6.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 20% (w/v) PEG 10000, 0.1M D-galactose, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
