3W5F
Crystal structure of tomato beta-galactosidase 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.820, 96.300, 159.267 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.090 - 1.650 |
| R-factor | 0.13113 |
| Rwork | 0.129 |
| R-free | 0.17589 |
| Structure solution method | SAD |
| Starting model (for MR) | 3d3a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.047 | 0.444 |
| Number of reflections | 170437 | |
| <I/σ(I)> | 33 | 3.8 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES, 20% (w/v) PEG 10000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
