3W58
Crystal structure of Galectin-1 in the lactose-unbound state(P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.461, 58.038, 93.762 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 37.630 - 1.580 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.630 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.068 | |
| Number of reflections | 99373 | |
| <I/σ(I)> | 11.28 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 277 | 2.0M ammonium sulfate, 1% 2-Mercaptoethanol, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
