3W56
Structure of a C2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.953697 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 25.348, 55.219, 89.746 |
Unit cell angles | 90.00, 91.17, 90.00 |
Refinement procedure
Resolution | 27.610 - 1.600 |
R-factor | 0.2137 |
Rwork | 0.212 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nsj |
RMSD bond length | 0.019 |
RMSD bond angle | 1.853 |
Data scaling software | SCALA (3.3.16) |
Refinement software | PHENIX (dev_895) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.027 | 44.864 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.048 | 0.355 | |
Rmeas | 0.054 | 0.399 | |
Rpim | 0.025 | 0.182 | |
Total number of observations | 2407 | 10548 | |
Number of reflections | 16003 | ||
<I/σ(I)> | 9 | 21.1 | 2.3 |
Completeness [%] | 97.9 | 99.5 | 96.5 |
Redundancy | 4.6 | 4.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 100mM Tris-HCl, 200mM MgCl2, 20% PEG 3350, pH 8.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |