3W43
Crystal structure of RsbX in complex with manganese in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.858, 48.925, 44.607 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.220 |
| R-factor | 0.1581 |
| Rwork | 0.157 |
| R-free | 0.18870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w45 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.260 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Number of reflections | 50676 | |
| <I/σ(I)> | 33.6 | 2.3 |
| Completeness [%] | 96.8 | 91.5 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 10% PEG 1000, 0.1M Tris-HCl, 3mM MnCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |
