3W42
Crystal structure of RsbX in complex with manganese in space group P1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 33.198, 41.649, 68.755 |
Unit cell angles | 81.17, 89.96, 71.46 |
Refinement procedure
Resolution | 20.000 - 1.060 |
R-factor | 0.12847 |
Rwork | 0.127 |
R-free | 0.15492 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w45 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.216 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.100 |
High resolution limit [Å] | 1.060 | 1.060 |
Number of reflections | 155995 | |
<I/σ(I)> | 23.6 | 2.3 |
Completeness [%] | 90.3 | 71.5 |
Redundancy | 3.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | 20 % PEG 1000, 0.1M Tris-HCl, 5mM MgCl2, 3mM MnCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |