3W40
Crystal structure of RsbX in complex with magnesium in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.081, 41.608, 69.038 |
| Unit cell angles | 81.00, 90.01, 71.14 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| R-factor | 0.1791 |
| Rwork | 0.176 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w45 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.779 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 75857 | |
| Completeness [%] | 90.0 | 68.4 |
| Redundancy | 2.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 20% PEG 1000, 0.1M Tris-HCl, 5mM MgCl2, pH 8.5, VAPOR DIFFUSION, temperature 298K |
