3W3Y

Crystal structure of Kap121p bound to Nup53p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-11
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	78.330, 131.440, 131.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.330 - 2.800
R-factor	0.25309
Rwork	0.251
R-free	0.29672
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w3t
RMSD bond length	0.011
RMSD bond angle	1.576
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.990	2.940
High resolution limit [Å]	2.800	2.800
Number of reflections	32341
Completeness [%]	95.2	79.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K