3W3X
Crystal structure of Kap121p bound to Pho4p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.090, 126.310, 128.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.500 - 2.900 |
R-factor | 0.22654 |
Rwork | 0.224 |
R-free | 0.26823 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w3t |
RMSD bond length | 0.011 |
RMSD bond angle | 1.532 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.970 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 28382 | |
Completeness [%] | 98.9 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |