3W3W
Crystal structure of Kap121p bound to Ste12p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.570, 126.160, 130.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.180 - 2.200 |
R-factor | 0.21733 |
Rwork | 0.215 |
R-free | 0.25622 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w3t |
RMSD bond length | 0.016 |
RMSD bond angle | 1.864 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.210 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 62953 | |
Completeness [%] | 95.9 | 75.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |