3W3V
Crystal structure of Kap121p mutant D353K/E396K/D438K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.790, 122.310, 84.670 |
| Unit cell angles | 90.00, 116.08, 90.00 |
Refinement procedure
| Resolution | 25.550 - 3.200 |
| R-factor | 0.2053 |
| Rwork | 0.203 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w3t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.542 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.550 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 23498 | |
| Completeness [%] | 99.6 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
