3W3U
Crystal structure of Kap121p mutant R349A/Q350A/D353A/E396A/N430K/D438A/N477A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.380, 124.200, 85.030 |
| Unit cell angles | 90.00, 116.73, 90.00 |
Refinement procedure
| Resolution | 26.540 - 2.600 |
| R-factor | 0.22409 |
| Rwork | 0.222 |
| R-free | 0.26737 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w3t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.771 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.550 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 43084 | |
| Completeness [%] | 97.7 | 84.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 10% 2-propanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
