3W3T
Crystal structure of Kap121p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.770, 124.390, 85.185 |
Unit cell angles | 90.00, 117.02, 90.00 |
Refinement procedure
Resolution | 28.320 - 2.900 |
R-factor | 0.20349 |
Rwork | 0.201 |
R-free | 0.24738 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w3z |
RMSD bond length | 0.013 |
RMSD bond angle | 1.788 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.100 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 31943 | |
Completeness [%] | 99.6 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 10% 2-popanol, 24% PEG 20000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |