3W3E
Structure of Vigna unguiculata chitinase with regulation activity of the plant cell wall
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.700, 48.600, 65.200 |
| Unit cell angles | 97.70, 100.40, 106.30 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.20386 |
| Rwork | 0.202 |
| R-free | 0.23493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dxj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.336 |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.900 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.062 |
| Number of reflections | 76333 |
| Completeness [%] | 92.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 30mg/ml protein, 0.1M Tris HCl, 0.1M Lithium sulfate, 30% PEG6000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
