3W3D
Crystal structure of smooth muscle G actin DNase I complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1991-02-15 |
| Detector | Macromolecular data collection system with a Weissenberg camera |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.000, 225.300, 77.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| Rwork | 0.196 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.340 |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 53428 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | precipitation | 6.6 | 300 | 4mM MgCl2, 0.1mM CaCl2, NaN3, PEG 6000, pH 6.6, precipitation , temperature 300K |
