3W3D
Crystal structure of smooth muscle G actin DNase I complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1991-02-15 |
Detector | Macromolecular data collection system with a Weissenberg camera |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.000, 225.300, 77.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
Rwork | 0.196 |
Structure solution method | MIR |
RMSD bond length | 0.016 |
RMSD bond angle | 3.340 |
Data reduction software | WEIS |
Data scaling software | WEIS |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 53428 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | precipitation | 6.6 | 300 | 4mM MgCl2, 0.1mM CaCl2, NaN3, PEG 6000, pH 6.6, precipitation , temperature 300K |