3W38
Sugar beet alpha-glucosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-30 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.534, 95.463, 107.651 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.670 - 2.790 |
R-factor | 0.2293 |
Rwork | 0.228 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.765 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.670 | 2.960 | |
High resolution limit [Å] | 2.790 | 8.240 | 2.790 |
Rmerge | 0.133 | 0.047 | 0.581 |
Rmeas | 0.052 | 0.645 | |
Number of reflections | 21842 | 943 | 3360 |
<I/σ(I)> | 12.13 | 26.82 | 3.64 |
Completeness [%] | 99.1 | 98.6 | 96.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100mM ammonium sulfate, 50mM sodium acetate buffer, 18% PEG monomethylether 2000, pH 4.5, vapor diffusion, hanging drop, temperature 298K |