3W35
Crystal structure of apo-type bacterial Vanadium-dependent chloroperoxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.711, 137.430, 156.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.810 - 2.400 |
| R-factor | 0.21446 |
| Rwork | 0.211 |
| R-free | 0.27165 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.810 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 49839 | |
| Completeness [%] | 97.6 | 93.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES-Na, 0.3M ammonium acetate, 8% PEG 8000, 2mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
