3W30
Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Collection date | 2012-01-01 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.583, 67.352, 84.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.990 |
R-factor | 0.23285 |
Rwork | 0.230 |
R-free | 0.30283 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b21 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.577 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.990 |
Rmerge | 0.141 |
Number of reflections | 8233 |
Completeness [%] | 100.0 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Magnesium Acetate, 0.1M Tris-HCl (pH6.5), 25% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |