3W2X

Crystal structure of DNA uridine endonuclease Mth212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-11-19
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	61.481, 61.481, 132.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.2144
Rwork	0.213
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fzi
RMSD bond length	0.009
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.073	0.049	0.598
Number of reflections	36324
<I/σ(I)>	13.5	23.2	4.8
Completeness [%]	97.0	74.3	100
Redundancy	10.7	7.7	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG3350, 50mM Magnesium formate dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K