3W2V
Crystal structure of the Cmr2dHD-Cmr3 subcomplex bound to 3'-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-10-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.940, 136.669, 191.973 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.730 - 2.600 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ung |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.063 | 0.063 |
Number of reflections | 42206 | |
Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 12.5-15% 2-propanaol, 18mM MgCl2, 45mM MES (pH 6.0), 20mM ammonium acetate, 10mM sodium acetate, 3% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |