3W2A
Crystal structure of VirB core domain complexed with the cis-acting site upstream icsp promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979590 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.316, 39.828, 73.491 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 48.246 - 2.775 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.26240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwb |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.924 |
| Data reduction software | XDS (package) |
| Data scaling software | XDS (package) |
| Phasing software | PHASER (MR) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.246 | 2.940 |
| High resolution limit [Å] | 2.775 | 2.780 |
| Number of reflections | 23902 | |
| <I/σ(I)> | 12.86 | 2.31 |
| Completeness [%] | 98.1 | 89.2 |
| Redundancy | 6.68 | 5.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 0.1M MgCl2 0.1M Sodium acetate 16% PEG-400, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
