3W1Y
Crystal structure of T brucei ATG8.2 in complex with E coli S10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.012, 61.197, 67.636 |
| Unit cell angles | 90.00, 110.77, 90.00 |
Refinement procedure
| Resolution | 44.880 - 2.300 |
| R-factor | 0.20521 |
| Rwork | 0.203 |
| R-free | 0.25649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h9d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.399 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.329 | |
| Number of reflections | 15654 | |
| Completeness [%] | 100.0 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG3350, MPD, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
