3W1I
Crystal structure of the N-terminal truncated selenocysteine synthase SelA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-03-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.07117 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 92.825, 280.936, 97.645 |
Unit cell angles | 90.00, 113.45, 90.00 |
Refinement procedure
Resolution | 48.941 - 3.192 |
R-factor | 0.1769 |
Rwork | 0.174 |
R-free | 0.23540 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.220 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.192 | 3.200 |
Rmerge | 0.090 | 0.674 |
Number of reflections | 75145 | |
<I/σ(I)> | 22.4 | 2.5 |
Completeness [%] | 99.5 | 96 |
Redundancy | 7.4 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 120mM Tris-HCl, 11% PEG 4000, 400mM CaCl2, 160mM KCl, 5.0mM NiCl2, 50mM Na2S2O3, 50mM L-serine, 0.2M NaCl, 10mM 2-mercaptoethanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
2 |