3W1E
Structure of FlgT, a flagellar basal body component protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9741 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.850, 34.150, 112.360 |
| Unit cell angles | 90.00, 102.43, 90.00 |
Refinement procedure
| Resolution | 27.960 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.990 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.210 |
| Number of reflections | 25447 | |
| <I/σ(I)> | 14.3 | 6.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.1M CHES-NaOH, 21-23% (v/v) PEG 3350, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
