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3W0O

Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia, ternary complex with ADP and hygromycin B

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-6A
Synchrotron sitePhoton Factory
BeamlineBL-6A
Temperature [K]95
Detector technologyCCD
Collection date2007-02-17
DetectorADSC QUANTUM 4r
Wavelength(s)0.9780
Spacegroup nameP 32 2 1
Unit cell lengths70.127, 70.127, 123.865
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution35.100 - 1.500
R-factor0.18536
Rwork0.184
R-free0.21178
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.018
RMSD bond angle1.734
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.6.0117)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]60.7501.580
High resolution limit [Å]1.5001.500
Number of reflections56794
Completeness [%]99.4100
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.22930.1M sodium chloride, 12% MPD, 0.1M sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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