3W0C
Crystal Structure Analysis of Vitamin D receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-01 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.370, 51.917, 132.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.190 - 1.900 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | CNX |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.863 | 27.863 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.080 | 0.360 | |
| Rmeas | 0.099 | 0.422 | |
| Rpim | 0.058 | 0.217 | |
| Total number of observations | 2206 | 11532 | |
| Number of reflections | 23148 | ||
| <I/σ(I)> | 6.7 | 9.3 | 3 |
| Completeness [%] | 93.5 | 89.1 | 89.3 |
| Redundancy | 3.5 | 2.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
