3W06
Crystal structure of Arabidopsis thaliana DWARF14 Like (AtD14L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.953, 55.603, 53.128 |
| Unit cell angles | 90.00, 115.81, 90.00 |
Refinement procedure
| Resolution | 47.830 - 1.150 |
| R-factor | 0.13313 |
| Rwork | 0.132 |
| R-free | 0.16210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w04 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 90920 | |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 95.9 | 93.1 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.0M AMMONIUM SULFATE, 0.2M BICINE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
