3W06
Crystal structure of Arabidopsis thaliana DWARF14 Like (AtD14L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.90 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.953, 55.603, 53.128 |
Unit cell angles | 90.00, 115.81, 90.00 |
Refinement procedure
Resolution | 47.830 - 1.150 |
R-factor | 0.13313 |
Rwork | 0.132 |
R-free | 0.16210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3w04 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.639 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Number of reflections | 90920 | |
<I/σ(I)> | 2.9 | |
Completeness [%] | 95.9 | 93.1 |
Redundancy | 3.6 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.0M AMMONIUM SULFATE, 0.2M BICINE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |