3VZU
Crystal Structure of outer membrane protein PorB from Neisseria meningitidis in complex with AMP-PNP
Replaces: 3A2UExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-31 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 |
| Unit cell lengths | 84.482, 84.482, 106.302 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.260 - 2.900 |
| R-factor | 0.23933 |
| Rwork | 0.237 |
| R-free | 0.27331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3A2R |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 8668 | |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 91.7 | 47 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 100mM MES, 50mM CsCl, 28-32% Jeffamine M-600, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
