3VZP

Crystal structure of PhaB from Ralstonia eutropha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	67.700, 123.690, 260.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.059 - 1.792
R-factor	0.1938
Rwork	0.192
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i01
RMSD bond length	0.007
RMSD bond angle	0.978
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.790	1.790
Number of reflections	100490
Completeness [%]	97.7	89.2
Redundancy	7.18	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	0.1M MES (pH 7.1), 1.6M ammonium sulfate, 10% 1,4-dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 293K