3VZH
Crystal Structure of CRISPR-associated Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-14 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.323, 43.781, 37.006 |
Unit cell angles | 90.00, 90.75, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.700 |
R-factor | 0.18522 |
Rwork | 0.183 |
R-free | 0.22613 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kg4 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.942 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 20990 | |
Completeness [%] | 96.5 | 95.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG3350, 0.2M sodium bromide, 0.1M Bis-Tris propane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |